Analysis of length distribution of TCRβ junctional sequences in cells from the mucosa of a bronchus–PLN–thymus cograft.

Shown are the profiles of cDNA amplified with pairs of Vβ–Cβ primers plus run-off with internal fluorescence Cβ primer. The percentage of cells expressing corresponding TCR Vβ, as estimated by flow cytometry, is indicated in the upper right corner of each histogram. Staining was considered significant when it was greater than 0.3%. According to this criterion, at least 12 of 24 distinct Vβ subsets were detected by flow cytometry. Immunoscope analysis suggested that the culture contained at least 40 distinct clonotypes.