Western blotting to detect cleaved forms of caspase 3 and PARP.

(A) HSC536N cells (C) and Jurkat cells (J) were treated with anti-Fas antibody for 6 hours and analyzed for the p20 and p17 cleaved forms of caspase 3 (top), and the p85 cleaved form of PARP (bottom). Note an artefact band migrating faster than p85 PARP is present in treated and untreated cells. (B) Jurkat cells were treated with etoposide for 6 hours and analyzed for the p20 and p17 cleaved forms of caspase 3 using Pharmingen antibody catalog no. 65906E1 or Transduction Laboratories antibody C31720.2