Schematic representation and nucleotide sequence of the VWF promoter.

(A) A schematic representation of the VWF promoter and the position of cis-acting elements and transacting factors that bind them are shown. The factors that function as repressors (NF1, Oct-1, and R?) are represented by circles and the factors that function as activators (Ets and GATA) are represented by rectangles. The TATA and CCAAT sequences are shown by filled and hatched rectangles, respectively. The arrow represents the position of the transcription initiation site. The expression pattern of various fragments of the promoter in endothelial and nonendothelial cells are shown. The + and − represent the promoter activity and lack of activity, respectively. (?) indicates unidentified. (B) The nucleotide sequence of the VWF promoter with the exact position of cis-acting elements is shown. The dotted line represents the sequence +215 to +247 that was shown to interact with protein complex “R.”