American Society of Hematology

Fig. 4.

$Fig. 4. The in vitro effects of IL-7 on TREC generation in TOCs. / Thymocytes from a newborn thymus were separated by MACS for CD3− and CD3+ subsets before or after 4 days of TOC in the absence or presence of IL-7 (10 and 50 ng/mL) and TREC levels were measured. (A) The representative data of CD3 expression is shown in CD3− (thin line) and CD3+ (bold line) fractions sorted by selection column from preculture and day 4 of TOC in the absence or presence of IL-7 (10 ng/mL). (B) TREC levels in the 2 fractions were measured. Three separate experiments were performed. Each experiment was performed in quadruplicate with all 3 concentrations of IL-7. A representative result is shown with error bars pertaining to the experiment.$

The in vitro effects of IL-7 on TREC generation in TOCs.

Thymocytes from a newborn thymus were separated by MACS for CD3⁻ and CD3⁺ subsets before or after 4 days of TOC in the absence or presence of IL-7 (10 and 50 ng/mL) and TREC levels were measured. (A) The representative data of CD3 expression is shown in CD3⁻ (thin line) and CD3⁺ (bold line) fractions sorted by selection column from preculture and day 4 of TOC in the absence or presence of IL-7 (10 ng/mL). (B) TREC levels in the 2 fractions were measured. Three separate experiments were performed. Each experiment was performed in quadruplicate with all 3 concentrations of IL-7. A representative result is shown with error bars pertaining to the experiment.

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