Fig. 2.
Proliferation rates and cell cycle stages during erythroid differentiation in vitro.
(A) Schematic representation of different erythroid stages during erythropoiesis as a percentage of the whole cell population. The dots mark the peak day for each cell type. Data collection and cell name abbreviations are as described in Figure 1. (B) Daily cell counts per 1 mL in culture. (C) Daily increase in cell counts measured as a percentage increase from the cell count on the previous day. (D) Flow cytometry measurements of cells at S, G2/M and G0/G1 stages of the cell cycle based on PI staining. (E) Representative cell cycle histograms performed on culture days 1, 8, and 13.