Fig. 1.
Glycosylation of scFv proteins expressed in
P pastoris. Culture supernatants containing scFv proteins derived from patients (F4, F5, and F16) and from the 5T33 mouse myeloma were separated by SDS-PAGE and visualized by Western blotting using 9E10 monoclonal antibody. The scFv proteins were either untreated (−) or treated (+) with PNGase.