American Society of Hematology

Fig. 3.

$Fig. 3. Specificity of antisera for murine NTPDase1 and NTPDase2. / Protein samples (5 μg) from COS cells, transiently transfected with NTPDase1 or NTPDase2 cDNA constructs or from cells transfected with empty vector DNA (as a negative control), were fractionated on 10% acrylamide SDS–polyacrylamide gel electrophoresis under nonreducing conditions. Separated proteins were then transferred to an Immobilon-P membrane and incubated with pAbs directed against NTPDase1 or NTPDase2. Each antiserum detected protein bands only in the appropriately transfected cells.$

Specificity of antisera for murine NTPDase1 and NTPDase2.

Protein samples (5 μg) from COS cells, transiently transfected with NTPDase1 or NTPDase2 cDNA constructs or from cells transfected with empty vector DNA (as a negative control), were fractionated on 10% acrylamide SDS–polyacrylamide gel electrophoresis under nonreducing conditions. Separated proteins were then transferred to an Immobilon-P membrane and incubated with pAbs directed against NTPDase1 or NTPDase2. Each antiserum detected protein bands only in the appropriately transfected cells.

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