Increased frequency of plasma cells in other E/P^−/− tissues.

ELISPOT analysis was performed to quantify plasma cell numbers in E/P^−/− peripheral blood, spleen, and bone marrow. (A) Four thousand leukocytes of each tissue type were added per well and each dot represents the mean number of antibody-secreting cells (ASCs) from triplicate wells for an individual mouse. For peripheral blood and spleen, wild-type (WT) n = 6 and E/P n = 14, and for bone marrow, wild-type n = 9 and E/P n = 19. The mean for each isotype is represented by a horizontal line. Note the scale difference for peripheral blood. (B) The total number of IgG-secreting plasma cells for wild-type versus E/P^−/− tissues is represented as the mean (± SEM) ASCs per 4000 input leukocytes per mouse. Error bars are present for each group, although SEM is too small to visualize for wild-type tissues and E/P^−/− bone marrow.