American Society of Hematology

Fig. 5.

$Fig. 5. Up-regulation of leukocyte adhesion molecules by plasma cells. / (A) Three-color flow cytometry analysis of the E/P−/−cervical lymph node B-cell populations (Figure 1) shows the relative expression of CD49d (α4), CD18 and CD11a (LFA-1), PSGL-1, L-selectin, and an isotype control antibody. The populations are as in Figure 1. A representative experiment of 6 is shown. (B) On the left the relative expression of CD44 on the 3 B-cell populations is shown. On the right a subset of purified plasma cells bind HA-FITC. A representative experiment of 8 is shown. HA-FITC staining is depicted on purified plasma cells (filled) versus the entire B-cell compartment (CD5−, Mac-1−; negative control, gray line). For the experiment shown, plasma cells constituted a small fraction of the entire B-cell compartment. An HA-binding T cell line, BW5147, was used as a positive control (dotted line).$

Up-regulation of leukocyte adhesion molecules by plasma cells.

(A) Three-color flow cytometry analysis of the E/P^−/−cervical lymph node B-cell populations (Figure 1) shows the relative expression of CD49d (α₄), CD18 and CD11a (LFA-1), PSGL-1, L-selectin, and an isotype control antibody. The populations are as in Figure 1. A representative experiment of 6 is shown. (B) On the left the relative expression of CD44 on the 3 B-cell populations is shown. On the right a subset of purified plasma cells bind HA-FITC. A representative experiment of 8 is shown. HA-FITC staining is depicted on purified plasma cells (filled) versus the entire B-cell compartment (CD5⁻, Mac-1⁻; negative control, gray line). For the experiment shown, plasma cells constituted a small fraction of the entire B-cell compartment. An HA-binding T cell line, BW5147, was used as a positive control (dotted line).

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