Effect of etodolac on the clearance of Indium 111 labeled patient leukocytes.

Two experiments are superimposed. One experiment without drug begun on March 21, 2000 and one with etodolac extended release administered 400 mg twice a day beginning at 7:30 am after blood collection for radiolabeling on November 8, 1999 and continued for 48 hours. Patient leukocytes were Indium 111 labeled according to supplier's instructions (Mediphysics, Amersham Healthcare, Arlington Heights, IL). Radiolabeled material left in the syringe after injection into the patient was submitted to the clinical laboratory at Scottsdale Healthcare Shea, Scottsdale, AZ for manual WBC differential counting. For the study of November 8, 1999 the radiolabeled cells were 100% lymphocytes and for the study of March 21, 2000, 67% were lymphocytes, 29% neutrophils, 3% eosinophils, and 1% bands. Indium-labeled patient leukocytes were injected at 11 am in both experiments. Total body WBC scans were performed in the Department of Radiology at Scottsdale Healthcare Shea using an ADAC Vertex Plus gamma camera (Milpitos, CA) under the supervision of Ronald Korn. The same camera was used for both studies. Scans were taken at 15 minutes, 2 hours, 24 hours, and 48 hours after injections and the percent of counts in the liver and spleen after background subtraction were determined at each time point. As the lymphocyte count dropped in response to etodolac administration, the percent of radiolabel in the spleen was increased in comparison to the percent of radiolabel in the spleen found without drug. No significant change in the percent of radiolabel in the liver was found with etodolac administration.