Transduction of Sca1⁺lin⁻ bone marrow cells and intracellular Notch1 expression in GFP⁺Sca1⁺lin⁻ bone marrow cells.

(A) Flow cytometric analysis for GFP in transduced Sca1⁺lin⁻ bone marrow cells 4 days after transduction. Plots represent fluorescence intensity for GFP on the x-axis and cell forward scatter on the y-axis. Numbers in corners indicate percent of events in that quadrant. (B) Flow cytometric analysis for intracellular Notch1 protein in GFP⁺Sca1⁺lin⁻ bone marrow cells. The polyclonal antibody directed against the intracytoplasmic region of Notch1 was used as described. Histograms are expressed as fluorescence intensity for Notch1 on the x-axis and cell count on the y-axis. Overlaid are fluorograms with anti-Notch1 antibody on MSCV-GFP cells (dotted line), anti-Notch1 on MSCV-ICN/GFP cells (thick solid line), and anti-Notch1 on MSCV-ΔE/GFP cells (thin solid line).