Mice receiving (1-210) bcr/abl-transduced BM cells develop MPD and B-ALL.
(A) FACS analysis of peripheral blood from a representative symptomatic wild-type P210 mouse at day 18 after BMT. (B) Some (1-210) bcr/abl mice develop MPD without evidence of B-ALL. FACS analysis of peripheral blood from a representative (1-210) bcr/abl mouse (no. 6) with MPD at day 28 after BMT. Note the similarity between this mouse and the wild-type P210 mouse in panel A. (C) The majority of (1-210) bcr/abl mice develop both MPD and B-ALL. FACS analysis of peripheral blood from a representative (1-210) bcr/abl mouse (no. 14) with both MPD and B-ALL at day 23 after BMT. Note the large population of B220+CD43+ cells in the GFP+fraction. (D) FACS analysis of lymph node tissue from the recipient of spleen cells from (1-210) bcr/abl mouse number 6. Note the abnormal populations of GFP+ immature B cells (CD43+B220+) and immature T cells (CD4+CD8+). For panels A through D, the gates used for separating the GFP− and GFP+populations are shown in the upper right panel. The lower panel pairs are divided into GFP− (left) and GFP+ (right) populations. The staining antibodies are adjacent to the axes. The percentage of cells in each population is indicated. The WBC count is measured per 106 cells/mL.
