Fig. 1.
PKC phosphorylation and proteolysis of adducin in thrombin-activated platelets.
Column-purified human platelets were exposed to dilute thrombin (0.1 U/mL) in the presence of 2.5 mM CaCl2 for 10 seconds to 5 minutes. (A) Coomassie blue–stained gel. (B) Western blotting with an antibody specific for the carboxy terminal PKC phosphorylation site of adducin demonstrated rapid phosphorylation of alpha and gamma adducins. Western blotting with a general anti-adducin antibody (C) demonstrated increasing proteolysis of adducin with time of exposure to thrombin.
