Western blot analysis after infusion of AAV vector.

Western blot analysis demonstrating presence or absence of anti-cFIX IgG in hemophilia B dogs Brad (A), Semillon (B), E34 (C), and Beech (D) as a function of time after AAV vector was infused. Numbers indicate weeks after vector administration. (E) Serum levels of anti-cFIX immunoglobulins in Beech as a function of time after vector administration. Note that no anti-cFIX was detected in Brad, Semillon, or E34.