Fig. 3.
Requirement of the coiled-coil domain of STAT5b-RARα for RARE binding and transcription activation.
(A) Schematic illustration of wild-type and mutated STAT5b-RARα constructs. (B) Gel-shift assays with wild-type and mutant STAT5b-RARα using 2 RAREs, DR5G and DR5T. (C) Transactivational activities of wild-type and mutant STAT5b-RARα in COS-7 cells. Following transfection with the indicated constructs, cells were cultured in medium with 10−6 M ATRA for 24 hours. Luciferase activity was normalized for transfection efficiency using a β-galactosidase reporter plasmid. The results presented are the mean ± SD of triplicate wells and are representative of 3 separate experiments.
