LCs from monkeys with AIDS undergo normal maturation in vitro.

Single-cell suspensions were generated from skin of a monkey with AIDS and labeled with mAb before and after 48 hours of culture with CD40L (3 μg/mL). (A) Cells were gated for expression of HLA-DR to identify LCs (R1). (B) Expression of surface antigens on freshly isolated LCs (closed histograms) and cultured LCs (open histograms). Representative histograms of 3 separate experiments using different animals are shown. Similar results were obtained with samples from healthy monkeys and monkeys with acute SIV infection.