ISH characterization of CXCL9/Mig mRNA in rhesus macaque lymphoid tissues during SIV infection.
Tissue sections from spleens (A, C, E, and G) and axillary lymph nodes (B, D, F, and H) were hybridized in situ with a35S-labeled, CXCL9/Mig-specific riboprobe, which was revealed by emulsion autoradiography after an exposure time of 7 days. (A,B) Macaque M5600 (uninfected), (C,D) macaque M5999 (acute infection), (E,F) macaque M6199 (AIDS), and (G,H) macaque M9597 (LTNP). The bar in panel A is equivalent to 500 μm. The inset in panel E represents ISH to a spleen tissue section from M6199 with a control sense riboprobe, shown at the same magnification. All images were captured as bright-field images, converted to grayscale, and then inverted to show the silver grains as bright white; gc indicates germinal center; rp, red pulp; pals, periarteriolar lymphoid sheath; pc, paracortex; and m, medulla. Original magnifications, × 100.
