Quantitation of CXCL9/Mig mRNA in lymph node tissues from rhesus macaques by image capture and analysis and real-time RT-PCR.

Quantitative image capture and analysis (A) and real-time RT-PCR (B) were used to determine CXCL9/Mig expression levels in lymph node specimens, as described in the legend for Figure 3. The levels of expression of IFN-γ mRNA as assessed by real-time RT-PCR are also presented in panel B. The 15 specimens obtained at necropsy are indicated by the asterisks in panel A. All differences in CXCL9/Mig expression measured by image capture and analysis were significant (P < .001) for the acute infection, clinical latency, and AIDS values compared with values in uninfected animals.