Reduced expression of CXCR3 on rhesus macaque peripheral blood lymphocytes during SIV infection.

Cryopreserved peripheral blood mononuclear cells (PBMCs) were stained for CXCR3 and CD3 or CXCR3 and CD8 and examined by using 2-color flow cytometry. Data are presented as the percentages of CD3⁺cells or CD8⁺ cells that were also CXCR3⁺. Mean values for each group are represented by black horizontal lines. Arrows indicate the values for M5599, which had only low-level viral replication. Open diamonds indicate uninfected or preinfection PBMCs gated on CD3⁺ lymphocytes; filled diamonds indicate uninfected or preinfection PBMCs gated on CD8⁺ lymphocytes; open circles indicate necropsy PBMCs gated on CD3⁺lymphocytes; filled circles indicate necropsy PBMCs gated on CD8⁺ lymphocytes. Comparisons of the percentage of CXCR3⁺ cells in uninfected and infected macaques, or paired comparisons of preinfection and necropsy time points showed significant differences only for CD3⁺ lymphocytes during acute infection (P = .005, 2 asterisks), although data for the CD8⁺ lymphocytes during acute infection were marginally different (P = .071, 1 asterisk).