Fig. 6.
Fig. 6. Bak-BH3 interaction with erythrocyte Bcl-XL. / Bak-BH3 peptides directly interact with erythrocyte Bcl-XL. Erythrocyte membrane extracts were incubated with 50 μM biotin-Ant or biotin-Ant–BH3 overnight at 4°C. The biotinylated peptides and any bound protein were then pulled down by incubating the extracts with streptavidin-coated agarose beads, and the presence of Bcl-XL was determined by Western blotting. FL5.12 cells were used as positive control for the coimmunprecipitation.

Bak-BH3 interaction with erythrocyte Bcl-XL.

Bak-BH3 peptides directly interact with erythrocyte Bcl-XL. Erythrocyte membrane extracts were incubated with 50 μM biotin-Ant or biotin-Ant–BH3 overnight at 4°C. The biotinylated peptides and any bound protein were then pulled down by incubating the extracts with streptavidin-coated agarose beads, and the presence of Bcl-XL was determined by Western blotting. FL5.12 cells were used as positive control for the coimmunprecipitation.

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