Fig. 7.
Effect of known caspases on Bak-BH3–induced erythrocyte cell death.
Bak-BH3–induced erythrocyte cell death occurs independently of known caspases. (A) First, 5 × 106 erythrocytes were incubated in SFM in the presence or absence of 80 μM zVAD. Following 15 minutes of incubation, 50 mM Ant or Ant-BH3 was added to the cell suspension. Cell number was determined at 0 and 24 hours in triplicate. Results are expressed as mean cell number ± SD and represent the 24-hour time point. (B) The α-Fas IgM–treated Jurkat cells were used as a control for zVAD. The percentages 21% and 1% represent the percentage of cells that have bound annexin-V FITC in the absence and presence of zVAD, respectively.
