Fig. 3.
Fig. 3. Rapid termination of CSF-1R signaling in ICSBP−/− BMMs. / BMMs were deprived of CSF-1 for 16 hours following stimulation with 200 ng/mL CSF-1 at 37°C for the indicated time points. Total extracts were subjected to SDS-PAGE and western blot analyses with an antiphospho-Erk-1/2 antibody (upper panel). To confirm equal protein loading the membrane was stripped and blotted with an anti–Erk-1/2 antibody (lower panel).

Rapid termination of CSF-1R signaling in ICSBP−/− BMMs.

BMMs were deprived of CSF-1 for 16 hours following stimulation with 200 ng/mL CSF-1 at 37°C for the indicated time points. Total extracts were subjected to SDS-PAGE and western blot analyses with an antiphospho-Erk-1/2 antibody (upper panel). To confirm equal protein loading the membrane was stripped and blotted with an anti–Erk-1/2 antibody (lower panel).

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