PKC inhibition blocks the antiapoptotic effect of TPA and IL-4.

B-CLL cells from 3 representative patients were incubated for 1 hour in the presence (▪) or the absence (■) of 5 μM BisI. After that time, drugs were added to the culture, incubation continued for another 48 hours. At the end of the culture, cell viability was analyzed by flow cytometry. Results are represented as the mean ± SD from one experiment performed in duplicate.