Fig. 6.
Effect of TPA and IL-4 on Akt phosphorylation.
Cells from 5 representative patients were incubated with 20 μM LY294002 for 1 hour before the addition of 10 nM TPA or 10 ng/mL IL-4 to the culture. Akt phosphorylation in Ser473 was analyzed by Western blot 20 minutes after the addition of drugs.