Survival analysis of developing lymphoid cells in Eμ-A1 transgenic mice.

(A) Thymocytes from Eμ-A1 transgenic or wild-type (WT) littermates (3 mice aged 5 weeks in each group) were cultured in vitro with medium alone, after γ-irradiation (2.5 Gy), or in the presence of 0.2 μM dexamethasone. Viability on the indicated days was ascertained by trypan blue exclusion. Representative results from 1 of 2 independent experiments are shown. (B) Survival analysis of B-lineage bone marrow cells. Bone marrow was cultured for 11 days and subjected to flow cytometric analysis for the indicated markers. Analysis is based on 100 000 events. Upper panels show light-scatter parameters on B220⁺ events and gate used to classify viable lymphoid cells. Lower panels show B220 and IgM expression on gated data. Percentages within each gate are shown. Data are representative of 2 experiments (the second was assayed after 12 days), each containing 2 or 3 mice of each genotype.