Effect of photodynamic cell purging process on anti–same-party and anti–third-party graft-versus-host disease.
Spleen cells from C57BL/6 mice were first primed with irradiated (20 Gy) BALB/c spleen cells in a 5-day mixed lymphocyte culture. The primed cells were then treated with photodynamic cell purging process. After treatment, 5 million cells were infused into lethally irradiated BALB/c (same party, 8.5 Gy) or C3H/HeJ (third party, 9.5 Gy) mice together with 1 × 107 T-cell–depleted bone marrow cells from C57BL/6 mice. Each groups contained 4 to 10 animals. Similar experiments have been repeated twice with similar results. Panels A and C represent body weight and survival of same-party BALB/c recipients, respectively; panels B and D represent body weight and survival of third-party C3H/HeJ recipients, respectively.
