Interaction of GATA-2 with FAZF.

(A) Schematic drawing of PLZF and FAZF. Percentages represent the homology between the indicated domains. (B) 293T cells transfected with an expression plasmid for GATA-2 in combination with an expression plasmid for Flag-tagged versions of PLZF, PLZF zinc fingers 7 to 9, FAZF, or FAZF zinc fingers. Cell lysates were prepared and immunoprecipitated (IP) with anti-Flag antibody. Immunoprecipitated materials were analyzed by Western blot analysis with anti–GATA-2 (top) and anti-Flag (bottom) antibodies. Input (10%) was used as a control for appropriate expression of the proteins indicated.