Interaction of GATA-2 with PLZF-RARα.

Nuclear extracts of 293T cells transfected with the indicated expression plasmids were immunoprecipitated with anti-Flag (A) or anti-PLZF (B) antibody and were analyzed by Western blotting with anti-Flag and anti-PLZF antibodies (A) or anti- PLZF and anti-GATA-2 antibodies (B). (C) Nuclear extracts of 293T cells transfected with expression plasmids for PLZF-RARα and Flag-tagged GATA-2 were incubated with biotinylated oligonucleotides harboring GATA motifs (wt GATA-oligo) or biotinylated mutant oligonucleotides in which GATA motifs were substituted with TTTA (mut GATA-oligo). Oligonucleotides were recovered by incubating with streptavidin–agarose beads, and precipitated proteins were analyzed by Western blotting with anti-PLZF or anti-Flag antibody.