Cryoglobulin, anti-IgG2a RF, and IgG3 self-associating activities of A6C, P11C, and Y12C IgG3 mAbs established from 6-19 heavy-chain transgenic mice.

(A) 0.1 mL of various concentrations of purified 6-19 (○), A6C (●), P11C (■), and Y12C (▴) mAbs were incubated for 48 hours at 4°C. Results are expressed concentrations of IgG3 in cryoprecipitates. Note the lack of cryoprecipitation of A6C, P11C, and Y12C mAbs, in contrast to the generation of significant amounts of cryoglobulins by the 6-19 mAb even at a concentration of 0.25 mg/mL. (B) Anti-IgG2a RF activity of purified 6-19 (○), A6C (●), P11C (■), and Y12C (▴) mAbs was determined by ELISA, and results are expressed as OD at 405 nm. (C) Various amounts of 6-19 (○), A6C (●), P11C (■), Y12C (▴), and 6-19 IgG1 variant, SS2F8 (▪), were incubated with¹²⁵I-labeled 6-19 mAb (10 ng) in the presence of 10 μL normal mouse serum. Results are expressed as a percentage of labeled 6-19 precipitated specifically.