American Society of Hematology

Fig. 4.

$Fig. 4. Separation of desialylated oligosaccharides of cryogenic 6-19, 6-19Q6E/K23A, and 6-19N471T, and noncryogenic A6C, P11C, and Y12C IgG3 mAbs by octadecylsilane-HPLC. / Desialylated ABEE-oligosaccharides of the IgG samples were separated into 8 oligosaccharide fractions which were eluted as a series of authentic biantennary complex-type oligosaccharide structures (positions 1 to 8) of ± Galβ1-4GlcNAcβ1-2Manα1-6(±Galβ1-4GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4(± Fucα1-6)GlcNAc shown in the right column. Peaks 1 and 5 correspond to digalactosylated (G2) oligosaccharide chains without or with a fucose residue; peaks 2, 3, 6, and 7 correspond to monogalactosylated (G1) oligosaccharide chains without or with a fucose residue; and peaks 4 and 8 correspond to nongalactosylated (G0) oligosaccharide chains without or with a fucose residue. G and Gal indicate galactose; GN and GlcNAc, N-acetylglucosamine; M and Man, mannose; F and Fuc, fucose.$

Separation of desialylated oligosaccharides of cryogenic 6-19, 6-19Q6E/K23A, and 6-19N471T, and noncryogenic A6C, P11C, and Y12C IgG3 mAbs by octadecylsilane-HPLC.

Desialylated ABEE-oligosaccharides of the IgG samples were separated into 8 oligosaccharide fractions which were eluted as a series of authentic biantennary complex-type oligosaccharide structures (positions 1 to 8) of ± Galβ1-4GlcNAcβ1-2Manα1-6(±Galβ1-4GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4(± Fucα1-6)GlcNAc shown in the right column. Peaks 1 and 5 correspond to digalactosylated (G2) oligosaccharide chains without or with a fucose residue; peaks 2, 3, 6, and 7 correspond to monogalactosylated (G1) oligosaccharide chains without or with a fucose residue; and peaks 4 and 8 correspond to nongalactosylated (G0) oligosaccharide chains without or with a fucose residue. G and Gal indicate galactose; GN and GlcNAc, N-acetylglucosamine; M and Man, mannose; F and Fuc, fucose.

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