Ability of BM-DCs from type I IFN-R KO mice to stimulate CD8⁺ and CD4⁺ T-cell proliferation.

BM-DCs from type I IFN-R KO mice exhibit a reduced ability to stimulate CD8⁺ and CD4⁺ T-cell proliferation. BM-DCs from type I IFN-R KO mice (dotted line [panels A-B] and open bars [panel C] or control mice (solid line [panels A-B]) and solid bars [panel C]) were harvested after 10 to 14 days of culture, irradiated, and used as APCs for TCR transgenic CD8⁺ (panels A-B) or CD4⁺ (panel C) T cells. (A) Different numbers of BM-DCs were added to culture wells together with 10⁵CD8⁺ responder T cells purified from 2C TCR transgenic mice. Cells were cultured with (open symbols) or without (solid symbols) specific peptide (Ser-Ile-Tyr-Arg-Tyr-Tyr-Gly-Leu at 0.05 nM). (B) Aliquots of 5000 BM-DCs were added per well together with 10⁵ purified CD8⁺ 2C T cells and the indicated concentration of specific peptide. (C) Different numbers of BM-DCs were added to culture wells together with 10⁵ CD4⁺responder T cells purified from DO11.10 TCR transgenic mice in the presence or absence of specific peptide (ovalbumin [OVA] aa 323 through 339 at 144 nM). Each of the panels shown here is representative of results obtained in at least 3 independent experiments.