Fig. 5.
Fig. 5. Expression of IFN-α by sDCs. / The sDCs were purified from B6 mice, incubated for 1 hour in medium containing GolgiPlug, placed on microscope slides, and fixed. In the upper row, cells were stained with rat antimouse IFN-α and biotinylated hamster antimouse CD11c followed by FITC-conjugated anti–rat immunoglobulin and Cy5-conjugated streptavin. Staining of cells in the middle row was done in the same manner, except that rat antimouse IFN-α was omitted, while in the lower row primary antibodies were replaced by isotype-matched controls. Cells were analyzed by confocal microscopy, where staining with FITC appears green. Cy5 staining was given a “false” blue color in the middle column and appears as a “true” red color in the right column. Colocalization of FITC and Cy5 staining appears yellow in the overlay. Magnification × 63 for all images.

Expression of IFN-α by sDCs.

The sDCs were purified from B6 mice, incubated for 1 hour in medium containing GolgiPlug, placed on microscope slides, and fixed. In the upper row, cells were stained with rat antimouse IFN-α and biotinylated hamster antimouse CD11c followed by FITC-conjugated anti–rat immunoglobulin and Cy5-conjugated streptavin. Staining of cells in the middle row was done in the same manner, except that rat antimouse IFN-α was omitted, while in the lower row primary antibodies were replaced by isotype-matched controls. Cells were analyzed by confocal microscopy, where staining with FITC appears green. Cy5 staining was given a “false” blue color in the middle column and appears as a “true” red color in the right column. Colocalization of FITC and Cy5 staining appears yellow in the overlay. Magnification × 63 for all images.

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