Effect of overnight culture of sDCs.

Overnight culture of sDCs induces phenotypic activation that is partially dependent on type I IFN. (A) Phenotype of CD11c⁺sDCs from B6 mice directly ex vivo (solid lines) and after overnight culture (dark-gray filled histograms). (B) Phenotype of CD11c⁺ B6 sDCs after culture for 18 hours in the presence (light-gray filled histograms) or absence (dark-gray filled histograms) of anti–IFN-α/β immunoglobulin. Dashed histograms show background staining with isotype control antibodies. Data are representative results from 1 of 3 experiments.