Localization of the H-Y epitope on the DBY protein between amino acids 164 and 209.

DBY protein and the DBY protein fragments indicated were added to irradiated HLA-DQ5⁺ female PBMCs. After incubation of 24 hours at 37°C, HLA-DQ5 HY CTLs were added; 16 hours later, supernatant was harvested and IFN-γ content was measured. Protein fragments containing residues at positions 67 to 209 and 164 to 308 in the DBY protein induced IFN-γ release by the HLA-DQ5 HY CTL.