Fig. 2.
Fig. 2. Establishment of Stat3F Tg mice. / (A) Schematic structure of the GATA-1 gene regulatory region (IE3.9int) cassette. This cassette contains 3.9-kb pairs of sequences 5′ to the IE exon, the IE exon itself, the first intron, and part of the second exon of the mouse GATA-1 gene. Stat3cDNA was linked to this cassette. (B) mRNAs were isolated from the spleen of the Tg mice, and the expression of Stat3F was verified by RT-PCR. Positions of the primer pairs are shown in Figure 2A. (C) High expression and dominant-negative regulation of Stat3F in the platelets from the Tg mice. Platelets were isolated from the Tg mice, and the expression of Stat3F was confirmed by Western blotting (lower panel). Stat3F worked as a dominant-negative regulator in TPO-induced tyrosine phosphorylation of Stat3 (upper panel).

Establishment of Stat3F Tg mice.

(A) Schematic structure of the GATA-1 gene regulatory region (IE3.9int) cassette. This cassette contains 3.9-kb pairs of sequences 5′ to the IE exon, the IE exon itself, the first intron, and part of the second exon of the mouse GATA-1 gene. Stat3cDNA was linked to this cassette. (B) mRNAs were isolated from the spleen of the Tg mice, and the expression of Stat3F was verified by RT-PCR. Positions of the primer pairs are shown in Figure 2A. (C) High expression and dominant-negative regulation of Stat3F in the platelets from the Tg mice. Platelets were isolated from the Tg mice, and the expression of Stat3F was confirmed by Western blotting (lower panel). Stat3F worked as a dominant-negative regulator in TPO-induced tyrosine phosphorylation of Stat3 (upper panel).

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