Delayed recovery of CFU-Meg from myelosuppression in Tg mice.

On day 7 after 5-FU injection, control mice (n = 3) and Stat3F Tg mice (n = 2) were killed. Isolated bone marrow cells were cultured with MegaCult-C media in the presence of 10 ng/mL IL-3, 20 ng/mL IL-6, and 50 ng/mL TPO. After 7-day culture, the colonies formed were stained with AChE. Results represent the mean number (±SD) of CFU-Meg colonies per femur.