Fig. 2.
Effect of PAF on adhesion of CHO-αIIbβ3-PAFR cells to immobilized Fg.
(A) Time course of the effect of PAF on adhesion of CHO-αIIbβ3-PAFR cells to immobilized Fg. CHO cells (4 × 105) coexpressing αIIbβ3 and PAFR were added to the wells of microtiter plates coated with 10 μg/mL Fg, incubated for different periods of time at 37°C in the presence or absence of 100 nM PAF, and visualized by phase contrast microscopy. (B) Comparative effect of PAF on adhesion of CHO, CHO-β3-PAFR, CHO-αIIbβ3, and CHO-αIIbβ3-PAFR cell lines to immobilized Fg. Cells were allowed to adhere on Fg-coated wells for 5 minutes, in the absence or presence of 100 nM PAF.
