Effect of PTX and PKC inhibitors on the effect of PAF in stimulating the adherence of CHO-αIIbβ3-PAFR cells to immobilized Fg.

(A) CHO-αIIbβ3-PAFR cells were incubated with 200 ng/mL PTX for 2 hours at 37°C and then stimulated with 100 nM PAF for 2 minutes. Cell adhesion to immobilized Fg at 10 minutes was measured as described in “Materials and methods.” To determine the effect of inhibiting PKC on the PAF-stimulated adhesion to immobilized Fg, cells were preincubated at 37°C with the indicated concentration of staurosporine (B) for 1 hour, H7 (C) for 45 minutes, or bisindolylmaleimide-I (BIM-I) for 30 minutes (D). The data are expressed relative to the adhesion of PAF-stimulated cells in the absence of inhibitor and are means ± SD of at least 3 experiments.