Effect of PAF on binding of soluble Fg or PAC1 to CHO-αIIbβ3-PAFR cells.

(A) Binding of FITC-Fg to CHO-αIIbβ3-PAFR cells. Cells were incubated for 15 minutes with soluble FITC-Fg, in the presence or absence of 100 nM PAF, as described in “Materials and methods.” The data are means of 3 independent experiments performed in duplicate. The insert shows the Scatchard plot of data. (B) The histograms represent the expression of PAC1 binding sites in cells incubated with or without 100 nM PAF.