American Society of Hematology

Fig. 3.

$Fig. 3. Effect of soluble agonists on membrane translocation of cytosolic NADPH oxidase components in COS-phoxcells. / COS-phox cells were activated with 0.4 μg PMA (10 minutes, 37°C) or 100 μM AA (5 minutes, 37°C), as indicated. Control cells were incubated for 10 minutes at 37°C with no stimulus. Cells were disrupted in a Dounce homogenizer and fractionated over a discontinuous 20%/38% sucrose gradient. The membrane fraction (6 μg) was separated by SDS-PAGE, transferred to nitrocellulose, and probed with antibodies directed against NADPH oxidase subunits Rac1, p47phox, p67phox, and p22phox. Blots are representative of 2 independent experiments.$

Effect of soluble agonists on membrane translocation of cytosolic NADPH oxidase components in COS-phoxcells.

COS-phox cells were activated with 0.4 μg PMA (10 minutes, 37°C) or 100 μM AA (5 minutes, 37°C), as indicated. Control cells were incubated for 10 minutes at 37°C with no stimulus. Cells were disrupted in a Dounce homogenizer and fractionated over a discontinuous 20%/38% sucrose gradient. The membrane fraction (6 μg) was separated by SDS-PAGE, transferred to nitrocellulose, and probed with antibodies directed against NADPH oxidase subunits Rac1, p47^phox, p67^phox, and p22^phox. Blots are representative of 2 independent experiments.

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