American Society of Hematology

Fig. 4.

$Fig. 4. Comparison of PKC isoform expression in COS-7 cells and human neutrophils. / Membrane (Mb) and cytosolic (Cyt) fractions were isolated from whole-cell lysates of human neutrophils or transgenic COS-7 cells expressing gp91phox/p22phox. Cell fractions (100 μg), along with protein standards for various PKC isoforms (50 ng), were separated by SDS-PAGE, transferred to nitrocellulose, and probed with PKC isoform-specific antibodies. The standard for PKC-ι was 13μg A431 cell lysate (Upstate). Representative blots are shown for PKC isoforms α (n = 2), βII (n = 3), δ (n = 3), ζ (n = 3), and ι (n = 3). The cytosolic fraction from wild-type COS-7 cells was also evaluated and gave results similar to those from the COS-7 cells expressing gp91phox/p22phox(not shown).$

Comparison of PKC isoform expression in COS-7 cells and human neutrophils.

Membrane (Mb) and cytosolic (Cyt) fractions were isolated from whole-cell lysates of human neutrophils or transgenic COS-7 cells expressing gp91^phox/p22^phox. Cell fractions (100 μg), along with protein standards for various PKC isoforms (50 ng), were separated by SDS-PAGE, transferred to nitrocellulose, and probed with PKC isoform-specific antibodies. The standard for PKC-ι was 13μg A431 cell lysate (Upstate). Representative blots are shown for PKC isoforms α (n = 2), βII (n = 3), δ (n = 3), ζ (n = 3), and ι (n = 3). The cytosolic fraction from wild-type COS-7 cells was also evaluated and gave results similar to those from the COS-7 cells expressing gp91^phox/p22^phox(not shown).

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