Confocal microscopic evaluation of DCs from 1- and 2-month cultures.

(A) DCs were purified from 1-month cultures by CD2-bead depletion of CTCL cells. The DCs were stained for cytoplasmic expression of the lysosomal marker LAMP (red) and class II MHC (green). The 2 images were merged to reveal colocalization of lysosomal compartments containing class II MHC (yellow). (B) DCs purified from 2- month cultures demonstrate class II colocalization (yellow) with LAMP (red) in lysosomal compartments and migration of class II molecules (green) to dendritic extensions of the cell membrane (arrow). (C) Fluorescent microscopic observation demonstrates cytoplasmic DC-LAMP staining (red) and membrane class II staining (green) on dendritic appendages of mature DCs with starfish morphology at 2 months of culture. Original magnification × 630.