SKY analysis of murine leukemias reveals a pattern of recurring numerical abnormalities.

(A-D). Metaphase cell from the spleen of a PML-RARA mouse with leukemia characterized by trisomy 15 as the sole abnormality (case 1950, 41,XY,+15). (A) Inverted image of the metaphase cell counterstained with 4,6-diamidino-2-phenylindole-dihydrochloride (DAPI). (B) Karyotype of the classified image. (C) Spectral image of the metaphase cell. (D) Karyotype of the spectral image. Trisomy for chromosome 15 is identified by a red box. (E-H). Metaphase cell from the spleen of a PML-RARA/BCL2 mouse with leukemia characterized by a hyperdiploid karyotype with trisomy for chromosomes 4, 6, 7, 12, 15, and 18 (case 67, 46,XY,+4,+6,+7,+12,+15,+18). (E) Inverted image of the DAPI-stained metaphase cell. (F) Karyotype of the classified image. (G) Spectral image of the metaphase cell. (H) Karyotype of the spectral image. Trisomic chromosomes are identified by a red box.