Indirect alkaline phosphatase staining on frozen sections of digestive biopsy specimens with histologic GVHD.

(A) In the infiltrate of the lamina propria, 2 of 3 labeled cells have bilobulated nuclei. Antibody directed against eosinophil peroxidase, × 300 magnification. (B) Staining is only found on inflammatory cells of the lamina propria, and not on epithelial glandular cells. Mononuclear and polynuclear cells are labeled. Antibody directed against IL-5, × 250. (C) Some inflammatory cells and mononuclear and polynuclear cells are stained in the inflammatory infiltrate of the lamina propria. Antibody directed against eotaxin, × 300 magnification.