Specific inhibition of dot blot assay by the addition of the truncated WT1 protein HWT3.

Dot blot assay was performed for a positive (black filled squares, empty squares, or gray filled squares) or negative (black filled diamonds, empty diamonds, or gray filled diamonds ) serum for IgG WT1 antibodies in the presence of increasing amounts of the truncated WT1 protein, HWT3 (closed symbols), human serum albumin (open symbols), or human transferrin (shaded symbols). Anti-WT1 polyclonal antibody, WT180 (black filled triangles, empty triangles, and gray filled triangles), was used as a positive control.