Fig. 1.
Proliferative effects of EphB4 on 32 cells.
(A) Expression of EphB4 in 3 sublines of 32D, EphB4 no. 5, EphB4 no. 8, and EphB4 no. 11, confirmed by Northern blot analysis. (B) EphB4 expression was analyzed by FACS using anti-EphB4 FITC-conjugated monoclonal antibody (right, dotted line). (C) Cell proliferation assay. Three 32D EphB4-positive sublines were cultured in the presence of 0.1 ng/mL IL-3 or 1% WEHI CM. Triplicate cultures of each subline were set at each condition at 10 × 104 cells/mL in a 24-well plate. Viable cells were counted and presented as the mean of 3 sublines. Similar results were obtained in 3 experiments. *P < .01.
