Effects of EphB4 overexpression on primitive cell compartment.

(A) LTC-IC assays were performed at week 6 from CD34⁺ cells transduced with MSCV-EphB4 or control. Data presented are from a representative experiment of 3 performed. (B) Reduction of CD34⁺CD38⁻ population in liquid culture by the transduction of EphB4. CD34⁺ cells were transduced with MSCV-GFP or MSCV-EphB4. GFP⁺ cells were cultured for 7 days. Two-color FACS was performed for the expression of CD34-PE and CD38-APC. Data shown represent the average of 5 experiments. Error bars represent standard deviation (P = .003).