![Fig. 1. Transduced CTLs surface express HA tag of mutant TGF-βRII receptor. / Cells of an EBV-specific CTL line 14 days after retroviral transduction with SFG:eGFP were stained with either PE-labeled anti-IgG1 (A) or anti-CD3 antibodies (B). CTLs transduced with SFG:HATGF-βRII-Δcyt (truncated TGF-βRII containing HA tag) gene were stained with FITC-labeled donkey antirabbit antibody alone (isotype control) (C). Nontransduced (D) or HATGF-βRII-Δcyt–transduced CTLs (E,F) were then stained with anti-HA monoclonal antibody followed by incubation with FITC-labeled donkey antirabbit antibody and PE-labeled CD8 or CD4 antibody. Six weeks after sorting CTLs for the HA tag, HA expression was measured on CD8+ cells (G [isotype control] and H). Surface immunofluorescence was analyzed by flow cytometry.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/9/10.1182_blood.v99.9.3179/6/h80922475001.jpeg?Expires=1735561971&Signature=gZNocicyS9cQ2fPKDjelz8NQCDBXtyc1p7gPGRAzbv5ctJXujILnH5fOkPF7l-JqdjqjuOv5dXlTv6fXKs3ZhADYBZ8imRUVu~1UWmgp2UgfLJXZMVmcjINbF~mRDjAwwKvCgJDLGsRiBK4VYSQsZ9gbczvQgOKSbxtNjc5I-kUfm-CyquViUnSwX1De6J54h1-bICW44ps-zxphm6vYK11xF18pyDcaBP5fxcdXgM66LVgTwbDzWgIg0lEt1RceJjU9qzxgqdochcXe5~ha~N-dBQFSa9iI3ZEE6YFrn1LMozwfpWISeGFeaw7u1xUM0SdXXjpxyBJYa1tnYSdUEg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transduced CTLs surface express HA tag of mutant TGF-βRII receptor.
Cells of an EBV-specific CTL line 14 days after retroviral transduction with SFG:eGFP were stained with either PE-labeled anti-IgG1 (A) or anti-CD3 antibodies (B). CTLs transduced with SFG:HATGF-βRII-Δcyt (truncated TGF-βRII containing HA tag) gene were stained with FITC-labeled donkey antirabbit antibody alone (isotype control) (C). Nontransduced (D) or HATGF-βRII-Δcyt–transduced CTLs (E,F) were then stained with anti-HA monoclonal antibody followed by incubation with FITC-labeled donkey antirabbit antibody and PE-labeled CD8 or CD4 antibody. Six weeks after sorting CTLs for the HA tag, HA expression was measured on CD8+ cells (G [isotype control] and H). Surface immunofluorescence was analyzed by flow cytometry.
