Low concentrations of sCD40L rescue L3055 cells from death arising from population depletion.
(Upper panel) L3055 cells (wild-type) were plated at the cell numbers indicated in flat-bottom microwells containing 200 μL culture medium in the presence of concentrations of sCD40L indicated. DNA synthesis was assessed 5 days later by 3HTdr incorporation during a 4-hour pulse. Results represent the mean (± SE) of 3 separate experiments and are expressed as cpm per 1000 cells plated. (Lower panel) L3055 cells (wild-type) were cultured at 104 per mL for 3 days with concentrations of sCD40L indicated and then stained with acridine orange to assess nuclear morphology and trypan blue to assess viability. Results are expressed as the mean percentage (with SE indicated) apoptotic (filled bars) and viable (open bars) cells of 3 separate experiments.
