Fig. 4.
Representative histograms of intracellular staining for Bcl-2 family members.
L3055 (wild-type) cells at 2 × 105 per mL were cultured for 3 days with sCD40L at 0, 0.2, or 20 ng/mL before analyzing by FACS for intracellular levels of Bcl-2, Bcl-xL, or Bax, as indicated. Histograms shown are as follows: 0 ng/mL (lighter shaded histogram), 0.2 ng/mL (light line), 20 ng/mL (heavy line). Appropriate isotype-matched control staining is indicated by darker shaded histogram for each protein detected and was essentially identical for cells harvested from each of the culture conditions.
